Hybridization method and composition with alternating polysaccha

Details Hybridization method and composition with alternating polysaccha Hybridization method and composition with alternating polysaccha

1989-08-31

1992-05-26

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 436501, 536 187, 536 21, 935 78, C12Q 168

Patent

active

051167271

ABSTRACT:
Probe cocktails contain a nucleic acid probe and a sulfated polysaccharide as a volume excluding polymer to speed up hybridization rates. Exemplary sulfated polysaccharides are chondroitin sulfate A and C, which can be extracted from cartilage and similar tissues. Compared to synthetic anionic polymers such as dextran sulfate, the probe cocktails can have reduced viscosities and surface tensions, as well as reduced water loss when the cocktail is heated to denature DNA before rehybridization. The reduced viscosity and surface tension are of particular value when entering and exiting narrow spaces, as in some automated analyzers and in capillary gap methodologies.

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A. I. Caplan, "Cartilage", Scientific American, vol. 251 (4) pp. 84-94 (1984).
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A. White et al, Principles of Biochemistry, pp. 53-54 (4th ed. 1968).
K. T. Montone et al, "Anatomic Viral Detection Is Automated" Yale J of Biol. & Med., vol. 62, pp. 141-158 (1989).

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