Hybridization-ligation assays for the detection of specific nucl

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 5, 435 4, 435 912, 536 243, 536 2433, 536 2436, C12Q 168, C12Q 170, C12P 1934, C07H 2104

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active

058009949

ABSTRACT:
A new method has been developed for conducting a gene probe assay. The preferred technique involves (1) using a gene amplification technique (e.g., PCR) to multiply the gene sequence of interest and (2) using a hybridization--ligation detection methodology, wherein the sequences of probes hybridized to the target sequence allow for separation and detection (e.g., probes might contain a combination of magnetic particles and acridinium esters) to determine if a specific sequence is present.

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