Hybridization incubator with rotisserie mechanism

Details Hybridization incubator with rotisserie mechanism Hybridization incubator with rotisserie mechanism

1990-06-01

1995-01-10

Housel, James C.

Chemistry: molecular biology and microbiology

Miscellaneous

435287, 435316, 435809, 422 99, 422104, 366214, 366217, C12M 110

Patent

active

053806620

ABSTRACT:
A hybridization incubator assembly (10) is provided for incubation of samples contained in elongated sample bottles (16) and short sample bottles (60). The incubator (10) includes and oven body (18) which encloses a rotisserie assembly (14) adapted for carrying and agitating the sample bottles. The rotisserie assembly (14) includes a pair of bottle support wheels (43) mounted eccentrically on a drive shaft (40). The first wheel (44) and the second wheel (46) are mounted at different degrees of eccentricity and are axially separated on the drive shaft (40) by a distance slightly less than the length of the elongated bottles (16). Each support wheel (43) includes a plurality of grasping clamps (60) affixed about the rim portion (56) for holding the bottles (16), with each the grasping clamps (60) being paired with another on the opposing wheel (43) such that a bottle (16) may be supported by both wheels (43). The perimeter surface (58) of each wheel is beveled to facilitate agitation. The drive shaft (40) is caused to rotate by a drive motor (36) and a drive linkage (38) which cause the rotisserie assembly (14) to rotate about a rotational axis (41), thus transporting and agitating the samples within the oven cavity (28). The incubator (10) is primarily utilized for nucleotide hybridization procedures.

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"The Controlled Environment Incubator," Technical Brochure of New Brunswick Scientific, N.J., 1966.
Fisher Scientific Catalog, 1988, pp. 630-631.
Bennahrnias, "An Alternative to the Water Bath/Plastic Bag Method for Hybridization of Southern and Northern Blots," American Biotechnology Laboratory, Sep. 1989.

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