Hybridization detection by pretreating bound single-stranded pro

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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422104, C12Q 168

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059855673

ABSTRACT:
An improved method for detecting hybridized biomolecules is provided which comprises the steps of, providing a plurality of probe biomolecules on a solid support surface; pre-treating the plurality of probe biomolecules with an effective amount of a pre-treatment solution to produce a signal having an increased intensity of at least two (2) times, as compared to an identical detection method that does not include the pre-treating step; applying a buffer solution to the probe biomolecules; hybridizing the probe biomolecules with a plurality of target biomolecules to form a hybridized complex; and, developing and detecting the signal intensity. The developing step of the method may further include the steps of, treating the hybridized complex with a conjugating solution, and treating the conjugated, hybridized complex with a detection reagent.

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