Hybridization assays on oligonucleotide arrays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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422 50, 422 681, 436501, 935 77, 935 78, C12Q 168

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active

060459964

ABSTRACT:
This invention provides methods of performing nucleic acid hybridization assays on high-density substrate-bound oligonucleotide arrays involving including in the hybridization mixture an isostabilizing agent, a denaturing agent or a renaturation accelerant.

REFERENCES:
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4652517 (1987-03-01), Scholl et al.
patent: 5015569 (1991-05-01), Pontius
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5324633 (1994-06-01), Fodor et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5521061 (1996-05-01), Bresser et al.
patent: 5632957 (1997-05-01), Heller et al.
Sambrook, J., et al., "Hybridization of Pools of Oligonucleotides", Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory press, 1989, pp. 11.48-11.51.
Khrapko, K.R., et al., "An oligonucleotide hybridization approach to DNA sequencing", FEBS Letters, 256:118-122, Oct. 1989.
Pontius, Brian W., "Close encounters: why unstructured, polymeric domains can increase rates of specific macromolecular association", TIBS, 18:181-186, May 1993.
Young, Richard A., et al., "Efficient isolation of genes by using antibody probes", Proc. Natl. Acad. Sci. USA, 80:1194-1198, Mar. 1983.
Melchior, William B., et al., "Alteration of the Relative Stability of dA-dT and dG-dC Base Pairs in DNA", Proc. Nat. Acad. Sci. USA, 70(2)298-302, Feb. 1973.
Pease, Ann Caviani, et al., "Light-generated oligonucleotide arrays for rapid DNA sequence analysis", Proc. Natl. Acad. Sci. USA, 91:5022-5026, May 1994.
Pontius, Brian W., et al., "Rapid renaturation of complementary DNA strands mediated by cationic detergents: A role for high-probability binding domains in enhancing the kinetics of molecular assembly processes", Proc. Natl. Acad. Sci. USA, 88:8237-8241, Sep. 1991.
Pontius, Brian W., et al. "Renaturation of complementary DNA strands mediated by purified mammalian heterogeneous nuclear ribonucleoprotein A1 protein: Implications for a mechanism for rapid molecular assembly", Proc. Natl. Acad. Sci. USA, 87:8403-8407, Nov. 1990.
Rees, William A., et al., Betaine Can Eliminate the Base Pair Composition Dependence of DNA Melting, Biochemistry, 32:137-144 (1993).
Van Ness, Jeffrey, et al., The use of oligodeoxynucleotide probes in chaotrop-based hybridization solutions, Nucleic Acids Research, 19(19):5143-5151 (1991).
Wetmur, James G., "DNA Probes: Applications of the principles of Nucleic Acid Hybridization", Critical Reviews in Biochemistry and Molecular Biology, 26(3/4):227-259 (1991).
Wood, William I., et al., "Base composition-independent hybridization in tetramethylammonium chloride: A method for oligonucleotide screening of highly complete gene libraries", Proc. Natl. Acad. Sci. USA, 82:1585-1588, Mar. 1985.
Nucleic Acid Hybridisation (Ed. Hames et al., IRL Press, Washington DC, 1985) pp. 6, 64, 65, 79, 190, and 191.

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