Hybridization assay and kit therefor

Details Hybridization assay and kit therefor Hybridization assay and kit therefor

1984-01-23

1986-09-23

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 436501, 436504, 436808, 935 2, 935 77, 935 78, C12Q 168, G01N 33566, G01N 33567

Patent

active

046135669

ABSTRACT:
A hybridization assay between two sets of parallel bands of DNA or RNA, one set labelled as a probe on a porous carrier sheet, the bands of the other set crossed with respect to the first and immobilized on a plurality of carrier sheets, is carried out with the sheets in contact and saturated with hybridization buffer in static condition. As many as ten sheets can be assayed simultaneously.

REFERENCES:
patent: 2783894 (1957-03-01), Lovell et al.
patent: 3876738 (1975-04-01), Marinaccio et al.
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4455370 (1984-06-01), Bartelsman et al.
patent: 4533628 (1985-08-01), Maas
Ranki et al., (1983), Gene 21: 77-85.
Masuda et al., (1979), Gene 6: 51-73.
Southern, J. Mol. Biol., vol. 98, 503-517, (1975).
Sato et al., PNAS, vol. 74, 542-546, (1977).
Wensink et al., Cell, vol. 18, 1231-46, (1979).

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