Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-08-16
2011-08-16
Whisenant, E C (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S022100, C536S023100, C536S024300, C435S091200
Reexamination Certificate
active
07998673
ABSTRACT:
A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm. The hybridisation probes of this invention have been demonstrated to accurately identify homozygous and heterozygous samples using a single fluorescent oligonucleotide and direct investigation of saliva with hybridisation probes permits ultra-rapid genotypic analysis within 35-40 minutes. Target detection and SNP discrimination assays have been achieved in homogeneous, heterogeneous, ‘real-time’ and solid-phase formats.
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Brown Tom
French David John
McDowell David Gordon
LGC Limited
Wenderoth , Lind & Ponack, L.L.P.
Whisenant E C
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