Hybrid tissue plasminogen activator/urokinase polypeptides

Details Hybrid tissue plasminogen activator/urokinase polypeptides Hybrid tissue plasminogen activator/urokinase polypeptides

1991-07-03

1993-04-20

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, 435226, C12N 948, C12N 964, C12N 972

Patent

active

052042553

ABSTRACT:
A hybrid plasminogen activator-like polypeptide comprising a polypeptide region responsible for an affinity to fibrin derived from a tissue plasminogen activator polypeptide and a polypeptide region responsible for an enzyme activity derived from a prourokinase polypeptide; a DNA segment coding for the hybrid polypeptide; plasmid containing the DNA segment; a microorganism transformed with the DNA; and a process for production of the hybrid polypeptide comprising culturing the microorganism and recovering the hybrid polypeptide from the cultured cells.

REFERENCES:
patent: 4752581 (1988-06-01), Robinson et al.
patent: 4753879 (1988-06-01), Rosa et al.
patent: 4992274 (1991-02-01), Robinson et al.
Van Zonneveld, A. et al., International Congress for the Society on Thrombosis & Haemostasis, San Diego, Ca. Abstract 022 from Talk, Jul. 15, 1985.
Van Zonneveld, A., et al., Proc. Natl. Acad. Sci., vol. 83, pp. 4760-4674, Jul. 1986.
Pennica, D. et al., Nature, vol. 301, pp. 214-221, 1983.
N.Y., T. et al., Proc. Natl. Acad. Sci., vol. 81, pp. 5355-5359, 1984.
Holmes, W. et al., Bio/Technology, vol. 3, pp. 923-929, Oct., 1985.
Gheysen, D. et al., J. Biol. Chem., vol. 262, pp. 11779-11784, Aug. 1987.
Pierardi, L. et al., J. Biol. Chem., vol. 262, pp. 11771-11778, Aug., 1987.
Nelles, L. et al., J. Biol. Chem., vol. 262, pp. 5682-5689, Apr., 1987.
Tor Ny, et al., "The Structure of the Human Tissue-Type Plasminogen Activator Gene: Correlation of Intron and Exon Structures to Functional and Structural Domains", Proc. Natl. Acad. Sci., vol. 81, pp. 5355-5359, Sep. 1984.
William E. Holmes, et al., "Cloning and Expression of the Gene for Pro-Urokinase in Escherichia Coli", Biotechnology, vol. 3, pp. 923-929 Oct. 1985.

Affiliated with

Also associated with

Rating

Hybrid tissue plasminogen activator/urokinase polypeptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hybrid tissue plasminogen activator/urokinase polypeptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybrid tissue plasminogen activator/urokinase polypeptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1525667