Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-09
1999-08-24
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 4352541, 4353201, 435325, 435366, 536 231, 536 245, C12Q 168, C12N 1563, C12N 1585, C07H 2104
Patent
active
059423951
ABSTRACT:
New hybrid ribozymes that exhibit the high metabolic stability of small nucleolar ribonucleic acids (snoRNAs), and that cleave target sequences with high efficiencies in either cis (intra-molecular) or trans (inter-molecular) configuration are described. The hybrid ribozymes include (i) a ribozyme catalytic sequence; and (ii) a snoRNA stabilizing motif; wherein the catalytic sequence and the stabilizing motif are arranged to provide the RNA molecule with a three-dimensional configuration in which the catalytic sequence is positioned to interact with a target sequence and the stabilizing motif adopts a conformation that stabilizes the RNA molecule, and additionally enables the RNA molecule to localize in the nucleolus of a cell.
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Cedergren Robert
Ferbeyre Gerardo
Fournier Maurille J.
Samarsky Dmitry A.
Elliott George C.
Universite de Montreal
University of Massachusettes
Wang Andrew
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