Hybrid recombinases for genome manipulation

Details Hybrid recombinases for genome manipulation Hybrid recombinases for genome manipulation

2005-04-29

2010-11-30

Raghu, Ganapathirama (Department: 1652)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

C435S455000, C435S462000, C435S069100, C435S320100, C435S091100, C435S183000, C536S023200, C424S094100

Reexamination Certificate

active

07842503

ABSTRACT:
The present invention provides methods to site-specifically manipulate genomes by using hybrid recombinases. Hybrid recombinases comprise a modified catalytic domain from a unidirectional serine phage integrase, fused to a foreign DNA recognition domain.

REFERENCES:
patent: 2002/0094516 (2002-07-01), Calos et al.
patent: 2004009792 (2004-01-01), None
patent: WO 2004/029233 (2004-04-01), None
Devos et al., Practical limits of function prediction. Proteins: Structure, Function, and Genetics. 2000, vol. 41: 98-107.
Kisselev L., Polypeptide release factors in prokaryotes and eukaryotes: same function, different structure. Structure, 2002, vol. 10: 8-9.
Nunes-Duby e al., Similarities and differences among 105 members of the Int family of site-specific recombinases. Nuc Acids Res., 1998, vol. 26(2): 391-406.
Whisstock et al., Prediction of protein function from protein sequence. Q. Rev. Biophysics., 2003, vol. 36 (3): 307-340.
Wishart et al., A single mutation converts a novel phosphotyrosine binding domain into a dual-specificity phosphatase. J. Biol. Chem., 1995, vol. 270(45): 26782-26785.
Witkowski et al., Conversion of b-ketoacyl synthase to a Malonyl Decarboxylase by replacement of the active cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650. Enablement.
Gregory et al., Integration site forStreptomycesphage phiBT1 and development of site-specific integrating vectors. J. Bacteriol., 2003, vol. 185 (17): 5320-5323.
Kuhstoss et al., Analysis of the integration function of the Sreptomycete bacteriophage phiC31. J. Mol. Biol., 1991, vol. 222: 897-908.
Akopian A et al., Chimeric recombinases with designed DNA sequence recognition: Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington D.C.; vol. 100 No. 15; Jul. 2003 pp. 8688-8691.
Gregory M. et al., “Integration site forStreptomycesphage ph1BTI and development of site-specific integrating vectors” Journal of Bacteriology; Sep. 2003 vol. 185, No. 17; pp. 5320-5323.
Groth, A.C., et al., “Phage Integrases: Biology and Applications”; Journal of Molecular Biology, London, GB; vol. 335, No. 3, Jan. 2004 pp. 667-678.
Shaikh A.C., et al. “Chimeras of the Flp and Cre recombinases: tests of the 18-23 mode of cleavage by flp and cre”; Journal of Molecular Biology, London, GB; vol. 302, No. 1; Sep. 2000 pp. 27-48.
Smith, M et al., Diversity in the Serine Recombinases:; Molecular Microbiology, Wiley-Blackwell Publishing Ltd, GB; vol. 44, No. 2; Jan. 2002 pp. 299-307.
Yagil, E. et al., “Identifying determinents of recombination specificity: Construction and characterization of chimeric bacteriophage integrases”; Journal of Molecular Biology; vol. 252, No. 2 (1995) pp. 163-177.

Affiliated with

Also associated with

Rating

Hybrid recombinases for genome manipulation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Hybrid recombinases for genome manipulation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybrid recombinases for genome manipulation will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4223288