Hybrid proteins produced by an ultrahigh prokaryotic expression

Details Hybrid proteins produced by an ultrahigh prokaryotic expression Hybrid proteins produced by an ultrahigh prokaryotic expression

1986-08-25

1989-12-19

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435320, 43525233, 435 694, 435 6951, 435 6952, 935 47, C12P 2102, C12N 1500, C12N 120, C12N 100

Patent

active

048882801

ABSTRACT:
Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host transformed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNZ and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.n express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject invention.

REFERENCES:
patent: 4617266 (1986-10-01), Fahnestock
patent: 4721671 (1988-01-01), Anilionis et al.

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