Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1984-12-26
1987-09-01
Phillips, Delbert R.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C07K 1500
Patent
active
046910093
ABSTRACT:
Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host transformed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNA and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.N express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject invention.
REFERENCES:
patent: 4322274 (1982-03-01), Wilson et al.
Chem. Abstr., vol. 104, (1986) 63133.
Waldman, A. S. et al. (1983), "Purification and Characterization of Herpes Simp. Virus (Type 1), Thymidine Kinase . . . ", J. Bact., 258:11571-11575.
Derom, C. et al. (1982), "High-Level Synthesis in E. coli of the SV40 Small-t Antigen Under Control of . . . ", Gene, 17:45-54.
Yoakum, G. H. et al. (1982), "Amplication of the uvrA Gene Product of E. coli to 7% of Cellular Protein by Linkage to the . . . ", Proc. Natl. Acad. Sci., 79:1766-1770.
Oehmichen, R. et al. (1984), "Construction of an E. coli Strain Overproducing the Tn10-Encoded TET Repressor and Its Use . . . ", EMBO J., 3:539-543.
Bagdasarian, M. (1983), "Activity of the Hybrid Trp-Lac(tac) Promoter of E. coli in Pseudomonas putida . . . ", Gene, 26:273-282.
Bikel, I. et al. (1938), "Purification of Biologically Active Simian Virus 40 Small Tumor Antigen", Proc. Natl. Acad. Sci. U.S.A., 80:906-910.
Amann, E. et al. (1984), "Vectors Bearing a Hybrid Trp-Lac Promoter Useful for Regulated Expression of Cloned Genes in E. coli", Gene, 25:167-178.
Shineberg, B. and Zipser, D. (1973), "The Ion Gene and Degradation of .beta.-Galactosidase Nonsense Fragments", J. of Bact., 116:1469-1471.
Chung, C. H. and Goldberg, A. L. (1981), "The Product of the Ion(capR) Gene in E. coli is the ATP-Dependent Protease . . . ", Proc. Natl. Acad. Sci., 78:4931-4935.
Sreedhara Swamy, K. H. and Goldberg, A. L. (1981), "E. coli Contains Eight Soluble Proteolytic Activities, One Being ATP Dependent", Nature, 292:652-654.
Mount, D. W. (1980), "The Genetics of Protein Degradation in Bacteria", Ann. Rev. Genet., 14:279-319.
Hautala, J. A. et al. (1979), "Increased Expression of a Eukaryotic Gene in E. coli through Stabilization of Its . . . ", Proc. Natl. Acad. Sci., 76:5774-5778.
Twigg, A. J. and Sherratt, D. (1980), "Trans-Complementable Copy-Number Mutants of Plasmid ColEl", Nature, 283:216-218.
Simons, G. et al. (1984), "High-Level Expression of Human Interferon Gamma in E. coli Under Control of the PL Promoter . . . ", Gene, 28:55-64.
DeBoer, H. A. et al. (1982), "Construction of a Tandem Trp-Lac Promoter and a Hybrid Trp-Lac Promoter for Efficient . . . ", Promoters: Structure and Function, 462-481.
Anilionis Algis
Palmer John L.
Phillips Delbert R.
Repligen Corporation
Saliwanchik David R.
Saliwanchik Roman
LandOfFree
Hybrid proteins produced by an ultrahigh prokaryotic expression does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hybrid proteins produced by an ultrahigh prokaryotic expression , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybrid proteins produced by an ultrahigh prokaryotic expression will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1265661