Hybrid proteins between an extracytoplasmic enzyme and at least

Details Hybrid proteins between an extracytoplasmic enzyme and at least Hybrid proteins between an extracytoplasmic enzyme and at least

1994-06-30

1996-07-09

Nucker, Christine M.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Sample mechanical transport means in or for automated...

435 79, 435 792, 435 21, 435975, 530350, 5303871, 5303913, 530399, G01N 33531

Patent

active

055342230

ABSTRACT:
A kit for carrying out an immunoenzymological assay for protein, containing a diagnostic reagent consisting of a hybrid protein comprising, in order, a polypeptide P1 which is an N-terminal portion of mature alkaline phosphatase comprising from 6 to 28 of the N-terminal amino acids of said mature alkaline phosphatase, joined to a polypeptide P2, capable of interacting with an antibody or antigen joined to a polypeptide P3 which is selected from the group consisting of a) the C-terminal remaining amino acids of said mature alkaline phosphatase, b) the mature sequence of alkaline phosphatase, and c) enzymatically active fragments of b).

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Kunapuei, S. P. et al. "Expression of Human Agiotensin . . . " J. Biol. Chem. 262 (16) 7672-75, 1987.
Duncancel et al. "Recombinant Colorimetric . . . " Bio/Technology 11:601-605, 1993.

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