Hybrid proteins between an extracytoplasmic enzyme and at least

Details Hybrid proteins between an extracytoplasmic enzyme and at least Hybrid proteins between an extracytoplasmic enzyme and at least

1992-04-30

1994-11-08

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 697, 4353201, 530350, 536 234, C07K 1300, C12N 1562

Patent

active

053626443

ABSTRACT:
The disclosed invention involves a DNA encoding a hybrid tripartite protein comprising an amino portion consisting of from 6 to 28 amino acid residues from the N-terminal of a mature E. coli alkaline phosphatase, a central portion corresponding to a heterologous polypeptide and a carboxyl portion which consists of the remainder of the mature E. coli alkaline phosphatase, a different alkaline phosphatase or an enzymatically active fragment of either. The tripartite proteins produced with this DNA can be used as a diagnostic reagent and in the screening of nucleic acid libraries or in the selection of recombinant clones.

REFERENCES:
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4769327 (1988-09-01), Stephens et al.
patent: 5011772 (1991-04-01), Recsei
Bio/Tech. 4:51-55, Jan. 1986, Matteucci et al. Alkaline Phosphatase Fusions: A Tag to Identify Mutations That Result in increased expression of secreted HGH from E. Coli.
Nucl. Acids Res. 17:10490, Dec. 1989, Obara et al. Sequence analysis of a cDNA encoding a erabutoxin b from the sea-snake Laticauda semifasciata.
PNAS 79:6598-6602, Nov. 1982, Gray et al Open reading frame cloning: Identification, cloning, and expression of open reading frame DNA.
J. Biol. Chem. 262:7672-7675, Jun. 5, 1987, Kunapuli et al Expression of Human Angiotensinogen cDNA in Escherichia coli.
EMBO J vol. 4:1075-1080, Apr. 1985, Nilsson et al Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.
Nature vol. 312:604-608, 13 Dec. 1984, Neuberger et al Recombinant antibodies possessing novel effector functions.
Gene vol. 73:409-418, 1988, Carbonell et al. Synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using . . . .
Biochemical and Biophysical Research, vol. 149, No. 2, 16 Dec. 1987, pp. 607-614.

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