Hybrid prokaryotic polypeptides produced by in vivo homologous r

Details Hybrid prokaryotic polypeptides produced by in vivo homologous r Hybrid prokaryotic polypeptides produced by in vivo homologous r

1989-11-01

1992-03-03

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435220, 435221, 435222, C12N 928, C12N 952, C12N 954, C12N 956

Patent

active

050932578

ABSTRACT:
Novel circular vectors containing a recplicable DNA sequence and DNA sequences encoding all or part of at least two distinct parental polypeptides are disclosed. Such vectors are used in novel processes utilizing in vivo recombination to produce recombined circular vectors containing said replicable DNA sequences and hybrid DNA sequences comprising: (1) a first DNA sequence encoding the amino-terminal portion of a hybrid polypeptide corresponding to a first part of a first parental polypeptide sequence and (2) a second DNA sequence encoding a carboxy-terminal portion of said hybrid polypeptide corresponding to a first part of a second parental polypeptide sequence. The hybrid DNA sequences of such recombined circular vectors can exprress novel hybrid polypeptides such as hybrid enzymes in general and in particular hybrid amylases and proteases. Various other processes are disclosed to isolate the recombined circular vector containing said hybrid DNA sequences.

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