Hybrid plasmids and microorganisms containing them

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 68, 435253, 435255, 435256, 435317, C12N 116, C12N 118, C12N 120, C12N 1500

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043871623

ABSTRACT:
A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2.mu. plasmid of yeast and a segment of DNA including the gene URA.sub.3.sup.+ of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.

REFERENCES:
Petes et al., "Isolation and Analysis of Recombinant DNA Molecules Containing Yeast DNA", Gene, 4 (1978), 37-49.
Bolivar et al., "Constructions and Characterization of New Cloning Vehicles I. Ampicillin-Resistant Derivatives of the Plasmid pMB9", Gene, 2 (1977), 75-93.
Hollenberg et al., Proc. Natl. Acad. Sci. U.S.A., 73(6), 2072-2076 (1976).
Hinnen et al., Proc. Natl. Acad. Sci. U.S.A., 75(4), 1929-1933 (1978).
Beggs, Nature, 275, 104-109 (1978).
Gerbaud et al., "2 .mu.m Plasmid Copy Number in Different Yeast Strains and Repartition of Endogeneous and 2 .mu.m Chimeric Plasmide in Transformed Strains", Current Genetics, 1, 219-228 (1980).

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