Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli co

Details Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli co Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli co

1979-08-21

1982-08-10

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435172, 435317, C12N 120

Patent

active

043439063

ABSTRACT:
A process for cloning DNA into a suitable host, which comprises fragmenting said DNA to obtain fragmented DNA, ligating said fragmented DNA into a suitable vector to obtain chimeric (hybrid) DNA, and transforming said chimeric DNA into said ultimate host. By this process, the useful chemical plasmid pUC3, which is obtainable from a biologically pure culture of the microorganism Streptomyces sp. 3022a, NRRL 11441, is cloned into the well-known bacterium E. coli HB101. This cloning of pUC3 into E. coli HB101 enables the production of large amounts of plasmid pUC3 DNA. pUC3 is useful as a cloning vehicle in recombinant DNA work. For example, using recombinant DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC3 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Malik, The Journal of Antibiotics, vol. XXX, No. 10, pp. 897-899, (1977).
Clarke et al., Proc. Natl. Acad. Sci., U.S.A., vol. 72, No. 11, pp. 4361-4365, Nov. 1975.
Maniatis et al., Cell., vol. 15, p. 687, Oct. 1978.

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