Hybrid plasmid and process of making same

Details Hybrid plasmid and process of making same Hybrid plasmid and process of making same

1981-03-02

1982-12-07

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 435849, C12N 1500, C12N 120, C12N 100, C12R 119

Patent

active

043628176

ABSTRACT:
A novel chemical compound, plasmid pUC1060, which was constructed from Streptomyces sp. 3022a chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC1060 contains a functional tet gene promoter composed of streptomycete and E. coli DNA, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC1060 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

REFERENCES:
Science, vol. 196, Apr., 1977-editorial.
Sutcliff, J. G., Nucleic Acids Research 5, 2721-2728, 1978.
Chakrabartz, Genetic Engineering CRC Press Inc. pp. 83 to 111 (1978).
Bolivar et al., Gene, vol. 2, pp. 95 to 113 (1977).
Sokaguchi et al., Molecular Breeding and Genetics of Microorganisms Academic Press Inc., pp. 130 to 137 (1980).
Francis et al., Con. J. Microbiol vol. 21, pp. 911 to 919 (1975).

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