Hybrid plasmid and process of making same

Details Hybrid plasmid and process of making same Hybrid plasmid and process of making same

1980-11-13

1982-12-07

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 435849, C12N 1500, C12N 120, C12N 100, C12R 119

Patent

active

043628168

ABSTRACT:
A novel chemical compound, plasmid pUC1031, which was constructed from Streptomyces sp. 3022a chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC1031 contains a functional tet gene promoter composed of streptomycete and E. coli DNA, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC1031 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

REFERENCES:
Chakrabarty, Genetic Engineering, CRC Press Inc. pp. 83 to 111 (1978).
Bolivar et al., Gene vol. 2 pp. 95-113 (1977).
Sakaguchi et al., Molecular Breeding and Genetics of Applied Microorganisms, Academic Press pp. 130-137 (1980).
Francis et al., Can. J. Microbiol. vol. 21 pp. 911-919 (1975).
Schrempf, H., Bujard, H., Hopwood, D. A. and Goebel, W. 1975, "Isolation of Covalently Closed Circular Deoxyribonucleic Acid from Streptomyces coelicolor A3(2), " J. Bacteriol. 121, 416-421.
Okanishi, M., Ohta, T. and Umezawa, H. 1969, "Possible Control of Formation of Aerial Mycelium and Antibiotic Production in Streptomyces by Episomic Factors, " J. Antibiotics 23, 45-47.

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