Hybrid plasmid and process of making same

Details Hybrid plasmid and process of making same Hybrid plasmid and process of making same

1980-06-16

1982-06-01

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 435849, C12N 1500

Patent

active

043328989

ABSTRACT:
A novel chemical compound, plasmid pUC1021, which was constructed from Bacillus megaterium chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC1021 contains a functional tet gene promoter, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC1021 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

REFERENCES:
Bolivar et al., Gene, 2 pp. 95-113 (1977).
Chakrabarty, Genetic Engineering, CRC Press Inc., pp. 83-111 (1978).

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