Hybrid neuronal cell lines compositions and methods

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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4351722, 4351723, 43524026, G01N 3353, C12N 516

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055145523

ABSTRACT:
The present invention provides a process of producing an immortalized hybrid neuronal cell comprising the steps of transducing a primary embryonic brain cell from a specific brain region with a temperature-sensitive oncogene to produce a transductant cell, and fusing the transductant cell with a mature primary neuron from the same brain region to produce the hybrid cell. Hybrid cells produced by such a process are also provided.

REFERENCES:
Eves et al. Soc. Neurosci. Abstracts 19:243 (1993).
Lee et al. Exptl Cell Res. 52:219-228 (1990).
Lendahl et al. TNJ 13:131-137 (1990).
Chol et al, Brain Res. 552:67-76 (1991).
Eves et al. Soc. Neurosci. Abs. 16:1149:(Abs. 475.2)(1990).
Jat et al. Mol. Cell Biol. 9:1672-1681 (1989).
Banker, G. and Goslin, K., In Culturing nerve cells (ed. G. Banker and K. Goslin), pp. 11-39. MIT Press, Cambridge, Massachusetts, 1991.
Birren, S. J. and Anderson, D. J., a v-myc- Immortalized Sympathoadrenal Progenitor Cell Line in Which Neuronal Differentiation Is Initiated by FGF but Not NGF, Neuron, 4:189-201, 1990.
Bottenstein, J. E., Differentiated Properties of Neuronal Cell Lines, In Functionally differentiated cell lines, (ed. G. Sato), pp. 155-184, Alan R. Liss, New York, 1981.
Eves, et al, Induction of Rapid Differentiation in Immortalized Rat Septal Cell Lines, Soc. Neurosci. Abstract, 17, 37, #21.5, 1991.
Eves, et al, Immortal Cell Lines From Embryonic Rat Hippocampal Precursor Cells, Soc. Neurosci. Abstract, 16, 1149, #475.2, 1990.
Frederiksen et al, Immortalization of Precursor Cells from the Mammalian CNS, Neuron, 1:439-448, 1988.
Giotta et al, Properties of Two Temperature-Sensitive Rous Sarcoma Virus Transformed Cerebellar Cell Lines, Brain Res., 202:445-458, 1980.
Goslin et al, Development of Neuronal Polarity: GAP-43 Distinguishes Axonal from Dendritic Growth Cones, Nature, 336:672-674, 1988.
Greene et al, Neuronal properties of hybrid neuroblastoma.times.sympathetic ganglion cells, Proc. Natl. Acad. Sci. USA, 72:(12)4923-4927, 1975.
Hammond et al, Development and Characterization of clonal cell lines derived from spetal cholinergic neurons, Brain Res., 512:190-200, 1990.
Hammond et al, Development and Characterization of Clonal Cell Lines Derived from Septal Cholinergic Neurons, Science, 234:1237-1240, 1986.
Morimoto, B. H. and Koshland, D. E., Jr., Excitatory amino acid uptake and N-methyl-d-aspartate-mediated secretion in a neural cell line, Proc. Natl. Acad. Sci. USA, 87:3518-3521, 1990.
Ronnett et al, Human Cortical Neuronal Cell Line: Establishment from a Patient with Unilateral Megalencephaly, Science, 248:603-605, 1990.
Schubert, D., In Developmental Biology of Cultured Nerve, Muscle, and Glia (ed. D. Schubert), pp. 1-25. John Wiley, New York, 1984.
Tucker et al, Expression of Epidermal Growth Factor Receptor in Immortalized Rat Hippocampal Cell Lines, Soc. Neurosci. Abstract, 16, 1149, #475.3, 1990.
Tucker et al, Expression of Epidermal Growth Factor Receptor in Immortalized Rat Hippocampal Cell Lines, FASEB J., 5, A7267, 1991.
Eves, et al., A Novel Hybrid Immortalization Strategy Yields a More Mature Neuronal Phenotype, Soc. Neurosci. Abstract, 19, 243, #107.3, 1993.

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