Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-08-22
2006-08-22
Wax, Robert A. (Department: 1653)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023500, C435S006120, C530S350000
Reexamination Certificate
active
07094888
ABSTRACT:
The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins ofCypridina luciferaseand synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.
REFERENCES:
patent: 5625048 (1997-04-01), Tsien et al.
De Angelis Dino
Miesenböck Gero
Rothman James E.
Mayer Suzanne M.
Memorial Sloan-Kettering Cancer Center
Morgan & Finnegan , LLP
Wax Robert A.
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