Hybrid gene regulatory region operable in E. coli

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 43525233, 4353201, 536 27, 935 41, C12N 1570, C12N 1573, C12N 121

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active

051622165

ABSTRACT:
This invention is directed to a novel hybrid regulatory region for directing and regulating transcription and translation of a gene sequence positioned downstream from the hybrid regulatory region. This hybrid regulatory region includes the promoter sequence of the phage lambda P.sub.R promoter-operator region fused to the operator sequence of the phage lambda P.sub.L promoter-operator region.

REFERENCES:
patent: 4551433 (1985-11-01), DeBoer
Botterman et al. (1987), DNA, vol. 6, pp. 583-591.
Ptashne et al. (1976), Science, vol. 194, pp. 156-161.
Maniatis et al., 1982 Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory pp. 17-21 and 405-406.
Herman A. DeBoer et al., "Construction of a Tandem, trp-lac Promoter and a Hybrid trp-lac Promoter," Promoters Structure and Function, 1982, Eds., Rodriguez et al.

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