Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-09-10
1998-04-14
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 711, 4352542, 4353201, 536 241, C12P 2106, C12N 119, C12N 1511, C12N 1581
Patent
active
057390070
ABSTRACT:
A hybrid yeast promoter comprising constituents of the 5' non-coding PGK region and, as an upstream activation sequence, the upstream activitation sequence of the GAL10 gene of Saccharomyces cerevisiae, and which does not contain the endogenous PGK upstream activation sequence. Preferably the GAL10 upstream activation sequence is provided at a site from which the PGK upstream activation sequence has been deleted. The hybrid promoter confers galactose regulation of gene transcription.
REFERENCES:
patent: 4803164 (1989-02-01), Hitzeman et al.
Guarente & Mason; "Heme Regulates Transcription of the CYC1 Gene of S. cerevisiae via an Upstream Activation Site" (1983) Cell 32, pp. 1279-1286.
Corraggio et al; "Transcription and expression of zein sequences in yeast under natural plant or yeast promoters" (1986) EMBO J 5, pp. 459-465.
Tuite et al; "Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae" (1982) EMBO J 1, pp. 603-608.
Mellor et al; "Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae" (1983) Gene 24, pp. 1-14.
Dobson et al; "Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae" (1982) Nucl Acids Res 10, pp. 2625-2637.
Guarente & Hoar; "Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the 'TATA box" (1984) PNAS USA 81, pp. 7860-7864.
Guarente et al. (1982), PNAS (USA), vol. 79: pp. 7410-7414.
Hawkins et al. (1982), Gene, vol. 19: pp. 55-58.
Bitter et al. (1984), PNAS (USA), vol. 81: pp. 5330-5334.
Cousens Diane Joan
Hinchliffe Edward
Kingsman Susan Mary
Wilson Mark Julian
Delta Biotechnology Limited
Elliott George C.
McKelvey Terry A.
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