Hybrid DNA conferring osmotic tolerance

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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4351723, 435253, 435317, 935 14, 935 29, 935 60, 536 27, C12P 1324, C12N 1500, C12N 120, C07H 1910

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active

045943230

ABSTRACT:
Non-conjugative episomes characterized by having a mutated proBA region are provided for conferring osmotic tolerance on osmotically sensitive hosts. The mutated DNA sequence overproduces at least one enzyme in the biosynthetic pathway for an amino acid which imparts the desired osmotic tolerance.
Cell lines E. coli CSH26 were deposited at the A.T.C.C. on Sept. 20, 1982 and given accession number 39202.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4278765 (1981-07-01), Debabove et al.

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