Hybrid cell lines producing monoclonal antibodies directed again

Details Hybrid cell lines producing monoclonal antibodies directed again Hybrid cell lines producing monoclonal antibodies directed again

1982-05-26

1985-04-30

Hazel, Blondel

Chemistry: molecular biology and microbiology

Spore forming or isolating process

260112R, 424 85, 424 87, 4351722, 935103, 935104, 935105, C12N 500, C12N 1500, C07G 700

Patent

active

045144982

ABSTRACT:
Continuous hybrid cell lines for producing monoclonal antibodies directed against antigens of Treponema pallidum have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with antigens of Treponema pallidum with hybridoma cells. The resulting fused cells were cloned, isolated, cultured and characterized as to antibody specificity against antigenic determinants of Treponema pallidum.

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patent: 4271145 (1981-06-01), Wands et al.
Kennett et al., "Hybrid Plasmacytoma Production: Fusions with Adult Spleen Cells, Monoclonal Spleen Fragments, Neonatal Spleen Cells and Human Spleen Cells", Lymphocyte Hybridomas, ed. by Melchers et al., Current Topics in Microbiology and Immunology, Springer-Verlag, Berlin, 1978, pp. 77-91.
Microbiology, Pelczar, Jr. et al., McGraw-Hill, New York, 1972, pp. 624-625.
Glass, David, "Medicine's Micro Missiles", Science Digest, 30-31:114, (Jan. 1982).
Miller, J. N., "Value and Limitations of Nontreponemal and Treponemal Tests in the Laboratory Diagnosis of Syphilis", Clinical Obstetrics and Gynecology, 18:191-203 (1975).

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