Hyaluronate synthase gene and uses thereof

Details Hyaluronate synthase gene and uses thereof Hyaluronate synthase gene and uses thereof

2005-02-08

2005-02-08

Wax, Robert A. (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S101000

Reexamination Certificate

active

06852514

ABSTRACT:
Disclosed are DNA sequences encoding hyaluronic acid synthase that are employed to construct recombinant cells useful in the production of hyaluronate synthase and hyaluronic acid (HA). In preferred aspects, chromosomal DNA encoding the HA synthase gene, hasA, was cloned from aStreptococcus pyogenesgenomic library. These vectors were used to transform host cells such asE. coliand acapsularStreptococcito produce hyaluronic acid. Resultant transformants were screened to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies were selected and employed in the production of hyaluronic acid.

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