Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2007-01-23
2007-01-23
Wax, Robert A. (Department: 1653)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S193000, C536S023200
Reexamination Certificate
active
11446854
ABSTRACT:
The present invention relates to a method of producing hyaluronic acid that includes introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into aBacillusstrain, and culturing theBacillusstrain under conditions appropriate for the production of hyaluronic acid.
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“Solubilization of Hyaluronic Acid Synthetic Activity from Streptococci and its Activation with Phospholipids”, Triscott et al., J. Biol. Chem., 261(13):6004-6009 (1986).
“Isolation of Streptococcal Hyaluronate Synthase”, Prehm et al., Biochem. J., 235:887-889 (1986).
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“The Isolation and Characterization of a Hyaluronidase Produced by a Capsulated Strain of C Streptococcus”, MacLennan, J. Gen. Microbiol., 14:143-152 (1956).
“The Biosynthesis of Hyaluronic Acid by Group A Streptococcus”, Markovitz et al., J. Biol. Chem., 234 (9):2343-2350 (1959).
“Biosynthesis of Hyaluronic Acid by Streptococcus”, Sugahara et al., J. Biol. Chem., 254:6252-6261 (1979).
“Streptococcal Hyaluronic Acid: Proposed Mechanisms of Degradation and Loss of Synthesis During Stationary Phase”, Van de Rijn, J. Bacteriol., 156(3):1059-1065 (1983).
“Solubilization of Hyaluronic Acid Synthetic Activity from Streptococci and its Activation with Phospholipids”, Triscott et al., J. Biol. Chem., 261(13):6004-6009 (1986).
“Isolation of Streptococcal Hyaluronate Synthase”, Prehm et al., Biochem. J., 235:887-889 (1986).
“Homologs of the Xenopus Developmental Gene DG42 are Present in Zebrafish and Mouse and are Involved in the Synthesis of Nod-Like Chitin Oligosaccharides During Early Embryogenesis”, Semino et al., Proc. Natl Acad. Sci. USA, 93:4548-4553 (1996).
“Enzymological Characterization of thePasteurella multocidaHyaluronic Acid Synthase”, DeAngelis,
DeAngelis Paul
Kumari Kshama
Weigel Paul H.
Dunlap Codding & Rogers P.C.
The Board of Regents of the University of Oklahoma
Wax Robert A.
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