Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-10-09
1999-10-19
Elliott, George P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 721, 435 74, 435 4052, 435366, C12Q 168, G01N 3353, G01N 33573, G01N 130
Patent
active
059687509
ABSTRACT:
Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.
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Hauswirth William W.
Muzyczka Nicholas
Zolotukhin Sergei
Elliott George P.
The University of Florida Research Foundation Inc.
Wang Andrew
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