Humanized green fluorescent protein genes and methods

Details Humanized green fluorescent protein genes and methods Humanized green fluorescent protein genes and methods

1997-07-16

2000-02-01

Degen, Nancy

Chemistry: molecular biology and microbiology

Vector, per se

4352351, 536 231, 536 235, C12N 1563, C07H 2104

Patent

active

060201924

ABSTRACT:
Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.

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