Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1996-01-18
1999-02-23
Degen, Nancy
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
4353201, 435325, 435354, 435357, 435358, 435365, 435367, 536 231, 536 235, C07H 2104, C12N 1563, C12N 1585
Patent
active
058743049
ABSTRACT:
Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.
REFERENCES:
patent: 5139941 (1992-08-01), Muzyczka et al.
patent: 5173414 (1992-12-01), Lebkowski et al.
patent: 5625048 (1997-04-01), Tsien et al.
Adams, Kondepudi, Gallagher, Kain, and Kitts, "Vectors for Using Green Fluorescent Protein (GFP) as a Reporter of Gene Expression and Protein Localization in Mammalian Cells" Abstract #465, in The FASEB Journal, 9(6):A1336, Apr. 1995, American Society for Biochemistry and Molecular Biology, San Francisco, California, May 21-25, 1995.
Bennetzen and Hall, "Codon Selection in Yeast," The Journal of Biological Chemistry, 257(6):3026-3031, Mar. 1982.
Brand, "GFP in Drosophila, " TIG, 11(8):324-325, Aug. 1995.
Chalfie, Tu, Euskirchen, Ward, and Prasher, "Green Fluorescent Protein as a Marker for Gene Expression," Science, 263:802-805, Feb. 1994.
Cheng and Kain, "Analysis of GFP and RSGFP Expression in Mammalian Cells by Flow Cytometry," CLONTECHniques, X(4):20, Oct., 1995.
Clontech Catalog, 1995-1996, pp. 156-160, Palo Alto, California.
Clontech, "Brighter, Red-Shifted Variants of GFP," CLONTECHniques, X(4):8-9, Oct., 1995.
Cody, Prasher, Westler, Prendergast, and Ward, "Chemical Structure of the Hexapeptide Chromophore of the Aequorea Green-Fluorescent Protein," Biochemistry, 32:1212-1218, 1993.
Cubitt, Heim, Adams, Boyd, Gross and Tsien, "Understanding, improving and using green fluorescent proteins," TIBS, 20:448-455, Nov., 1995.
Delagrave, Hawtin, Silva, Yang and Youvan, "Red-Shifted Excitation Mutants of the Green Fluorescent Protein," Bio/Technology, 13:151-154, Feb., 1995.
Grantham, Gautier, Gouy, Mercier, and Pave, "Codon catalog usage and the genome hypothesis," Nucleic Acids Research, 8(1):r49-r63, 1980.
Grantham, Gautier, Gouy, Jacobzone, and Mercier, "Codon catalog usage is a genome strategy modulated for gene expressivity," Nucleic Acids Research, 9(1):r43-r75, 1981.
Haselhoff and Amos, "GFP in plants," TIG, 11(8):328-329, Aug., 1995.
Heim, Cubitt and Tsien, "Improved green fluorescence," Nature, 373:663-664, Feb., 1995.
Heim, Prasher, and Tsien, "Wavelength mutations and posttranslational autoxidation of green fluorescent protein," Proc. Natl. Acad. Sci USA, 91:12501-12504, Dec., 1994.
Hodgkinson, "GFP in Dictyostelium," TIG, 11(8):327-328, Aug., 1995.
Ikemura, "Correlation between the Abundance of Escherichia coli Transfer RNAs and the Occurrence of the Respective Codons in its Protein Genes: A Proposal for a Synonymous Codon Choice that is Optimal for the E. coli Translational System," J. Mol. Biol., 151:389-409, 1981.
Ikemura, "Correlation Between the Abundance of Yeast Transfer RNAs and the Occurrence of the Respective Codons in Protein Genes," J. Mol. Biol., 158:573-597, 1982.
Ikemura, "Correlation between the Abundance of Escherichia coli Transfer RNAs and the Occurrence of the Respective Codons in its Protein Genes," J. Mol. Biol., 146:1-21, 1981.
Inouye and Tsuji, "Aequorea green fluorescent protein Expression of the gene and fluorescence characteristics of the recombinant protein," FEBS Letters, 341:277-280, 1994.
Morin and Hastings, "Energy Transfer in a Bioluminescent System," J. Cell. Physiol., 77:313-318, 1971.
Prasher, Eckenrode, Ward, Prendergast and Cormier, "Primary structure of the Aequorea victoria green-fluorescent protein," Gene, 111:229-233, 1992.
Prasher, "Using GFP to see the light," TIG, 11(8):320-323, Aug. 1995.
Rizzuto, Brini, Pizzo, Murgia and Pozzan, "Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells," Current Biology, 5(6):635-642, 1995.
Shimomura, "Structure of the Chromophore of Aequorea Green Fluorescent Protein," FEBS Letters, 104(2):220-222, Aug., 1979.
Wada, Aota, Tsuchiya, Ishibashi, Gojobori, and Ikemura, "Codon usage tabulated from the GenBank genetic sequence data," Nucleic Acids Research, 18(Suppl.):2367-2411, 1990.
Wang and Hazelrigg, "Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis," Nature,369:400-403, Jun., 1994.
Ogawa et al., "Localization, Trafficing, and Temperature-Dependence of the Aequorea Green Fluorescent Protein in Cultured Vertebrate Cells," Proc. Natl. Acad. Sci. USA, 92:11899-11903, Dec., 1995.
Hawley-Nelson et al., "Rapid, High Level Detection of Mammalian Cell Transfection with pGreen Lantern--1, a Humanized Mutant GFP Reporter Vector," FASEB Journal, 10(6):A1124, abstract #727, 1996.
Life Technologies Incorporated, "Optimized Transfections Using New Cationic Lipid Reagents and a Mutant `Humanized` GFP Transfection Reporter Vector," FASEB Journal 10(6):A1529, abstract #T22, 1996.
Levy J. P. et al., "Retroviral Transfer and Expression of a Humanized Red-Shifted Green Fluorescent Protein Gene into Human Tumor Cells," Nature Biotechnology, 14(5):610-615, 1996.
Zolotukhin et al., "A `Humanized` Green Fluorescent Protein cDNA Adapted for High-Level Expression in Mammalian Cells," J. Virol., 70(7):4646-4654, 1996.
Wang, et al., "The Pleckstrin Homology Domain of Human .beta.I.SIGMA.II Spectrin is Targeted to the Plasma Membrane in Vivo," Biochemical and Biophysical Research Communications, 225:420-426, 1996.
Hauswirth William W.
Muzyczka Nicholas
Zolotukhin Sergei
Degen Nancy
University of Florida Research Foundation Inc.
Wang Andrew
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