Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Patent
1997-12-09
2000-09-26
Ungar, Susan
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
530350, 530828, 530834, 514 2, 514 12, 514 21, A61K 3846, A61K 3800, C07K 100
Patent
active
061239380
ABSTRACT:
The present invention is based on the purification and sequencing of isozymes of plasma hyaluronidase (pHAse) found in urine. Specifically, urine contains two hyaluronidases (HAses): 1) a 57 kDa HAse that is apparently the same as the 57 kDa HAse found in plasma; and 2) a 45 kDa HAse, which is found in urine but not plasma. The smaller urine isozyme is composed of two disulfide-linked polypeptides produced by endoproteolytic cleavage of the 57 kDa isoform. The present invention thus features a urinary hyaluronidase (uHAse) polypeptide and nucleotide sequences encoding a Chain A polypeptide and a Chain B polypeptide, the two polypeptides of which uHAse is composed. In a particular aspect, the uHAse is a human uHAse (huHAse), preferably a huHAse composed of the Chain A and B polypeptides having SEQ ID NOS: 2 and 4, respectively. In related aspects the invention features polynucleotide sequence encoding Chain A and Chain B polypeptides, preferably having the sequences of SEQ ID NOS: 1 and 3, respectively. In addition, the invention features polynucleotide sequences that hybridize under stringent conditions to SEQ ID NOS: 1 and 3. In related aspects the invention features expression vectors and host cells comprising polynucleotides that encode uHAse polypeptide Chains A and B. The present invention also features antibodies that bind specifically to uHAse, and methods for producing uHAse.
REFERENCES:
Reiger et al (Glossary of Genetics and Cytogenetics, Classical and Molecular, 4th Ed., Springer-Verlag, Berlin, p. 17), 1976.
Frost, et al., "Purification, Cloning, and Expression of Human Plasma Hyaluronidase," Biochemical and Biophysical Research Communications 236:10-15 (1997).
Csoka Anthony
Frost Gregory I.
Stern Robert
Wong Tim M.
Francis Carol L.
The Regents of the University of California
Ungar Susan
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