Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1989-11-02
1992-05-05
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530350, 530413, 530810, 530828, 436 15, 436 87, 436545, C07K 1502, C07K 1514, G01N 3348, G01N 33543
Patent
active
051109115
ABSTRACT:
Human tumor associated Thomsen-Friedenreich (TF) antigen is purified from adenocarcinoma conditioned media, adenocarcinoma cell detergent extracts or plural effusion fluid by affinity chromatography using an insolubilized TF-specific monoclonal antibody, MAb 49H.8. The TF antigen is a glycoprotein characterized by a non-cryptic Gal .beta.(1.fwdarw.3) GalNAc epitope, a molecular weight in excess of 1,000,000 daltons, and extractability with perchloric acid, the epitope being sensitive to alkali and periodate but resistant to acid. A heterologous sandwich immunoassay has been developed for human TF antigen using a monoclonal antibody as the catcher and labelled peanut agglutinin as the probe. Since human TF antigen is shed by tumor cells, a positive determination of the TF antigen in a patient sample indicates the presence of cancer. The test can also be used to monitor the course of conventional chemotherapy or radiotherapy by monitoring the amount of TF antigen in a fluid sample from a patient being so treated. Because of the sensitivity of the assay method, cancer can be detected in very early stages by the assay method of the present invention.
REFERENCES:
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Gold and Miller, Nature 255: 85-87, 1 May 1975.
Boland et al., Proc. Natl. Acad. Sci. U.S.A. 79:2051-2055, Mar. 1982.
Springer et al., Science 224:1198-1206 (1984); T and Tn, General Carcinoma Autoantigens.
Samuel et al., Cancer Research, 50:4801-8 (1990); Analysis of Human Tumor Associated Thomsen-Friedenreich Antigen.
Itzkowitz et al., Cancer Research, 49: 197-204; (1989), Expression of Tn, Sialosyl-Tn, and T Antigens in Human Colon Cancer.
Longenecker B. Michael
Samuel John
Biomira, Inc.
Cooper Iver P.
Walsh Stephen
Wax Robert A.
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