Human thrombopoietin comprising glycosylation sited at...

Drug – bio-affecting and body treating compositions – Lymphokine

Reexamination Certificate

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C530S395000, C530S351000, C435S069500, C435S358000, C435S320100

Reexamination Certificate

active

07037491

ABSTRACT:
The present invention relates to novel human thrombopoietin (hTPO) derivatives, and to process of preparation thereof. Particularly, sugar chains are introduced into native hTPO by substituting amino acids such as asparagine for amino acids at specific positions in native hTPO, preparing novel hTPO derivatives with high activities enhancing the platelet production in vivo. Therefore, the novel hTPO derivatives of this invention may be useful for the treatment of thrombocytopenia associated with anticancer therapy or the transplantation of bone marrow.

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patent: WO 96/25498 (1996-08-01), None
K. H. Pearce, Jr., et al., “Mutational analysis of thrombopoietin for identification of receptor and neutralizing antibody sites”, Journal of Biological Chemistry 272(2): 20595-20602, Aug. 15, 1997.
Hoffman et al., “Peptide, Disulfidc, and Glycosylation Mapping of Recombinant Human Thrombopietin Ser 1 to Arg 246”,Biochemistry, 1996, 35(47), 14849-14861 (Eng.) Columbus, OH, USA: Chemical Abstracts, vol. 126, No. 6, Feb. 10, 1997, p. 80, Abstract No. 70279s.
Park et al., “Identification of Funtionally Important Residues of Human Thrombopoietin”,The Journal of Biological Chemistry, vol. 273, No. 1, Jan. 2, 1998, pp. 256-261.
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