Human synthetic single-chain antibodies directed against the...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S387700, C530S391100

Reexamination Certificate

active

08039594

ABSTRACT:
Isolated polypeptides, isolated polynucleotides or expression vectors encoding same, viral display vehicles which can be specifically bind an exposed epitope shared by mutant, but not wild type, p53 protein are provided. Also provided are methods of inducing apoptosis and treating cancer as well as diagnosing a p53-related cancer using the isolated polypeptides uncovered by the present invention.

REFERENCES:
patent: WO 98/18825 (1998-05-01), None
patent: WO 01/68801 (2001-09-01), None
Cellular and Molecular Immunology (Eds. Abass et al.; 1991; W.B. Saunders: Philadelphia; p. 54).
Azriel-Rosenfeld et al. “A Human Synthetic Combinatorial Library of Arrayable Single-Chain Antibodies Based on Shuffling In Vivo Formed CDRs Into General Framework Regions”, Journal of Molecular Biology, 335(1): 177-192, 2004.
Gannon et al. “Activating Mutations in P53 Produce A Common Conformational Effect. A Monoclonal Antibody Specific for the Mutant Form”, The EMBO Journal, 9(5): 1595-1602, 1990.
Govorko et al. “Single-Chain Antibody Against the Common Epitope of Mutant P53: Isolation and Intracytosolic Expression in Mammalian Cells”, Journal of Immunological Methods, 258: 169-181, 2001.
Orgad et al. “Single Chain Antibody Against the Common Epitope of Mutant P53 Restores Wild-Type Activity to Mutant P53 Protein”, FEBS Letters, 579(25): 5609-5615, 2005.
Communication Relating to the Results of the Partial International Search Dated Aug. 4, 2006 From the International Searching Authority Re.: Application No. PCT/IL2006/000372.
International Preliminary Report on Patentability Dated Oct. 4, 2007 From the International Bureau of WIPO Re.: Application No. PCT/IL2006/000372.

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