Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Animal or plant cell
Patent
1993-06-15
1997-09-30
Elliott, George C.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Animal or plant cell
A61K 3514
Patent
active
056723466
ABSTRACT:
Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.-, and c-kit receptor positive (KR.sup.+) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.- and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.
REFERENCES:
Sustained human hematopoiesis in sheep transplanted in utero during early gestation with fractionated adult human bone marrow cells. Srour et al. Blood Mar 15 1992, 79 (6) pp. 1404-1412.
Linch et al. (1986) The Lancet Dec. 20/27: p. 1453.
Palavicini et al. (1991) Clinical Research 39(2):378A.
Fleishman and Mintz (1979) P.N.A.S. 76(11):5736-5740.
Fleishman and Mintz (1984) J. Exp. Med. 159:731-745.
Diukman and Golbus (1992) Journal of Reproductive Medicine 37(6):515-520.
Slavin et al. (1992) Bone Marrow Transplant. 1:189-190.
Touraine et al.; Transplantation Proceedings, vol. 21, No. 1; pp. 3112-3113; Feb. 1989.
Srour et al.; Cytometry, vol. 12, pp. 179-183; 1991.
Srour et al.; Blood Cells, vol. 17, pp. 287-295; 1991.
Briddell et al.; Blood, vol. 79, No. 12, pp. 3159-3167; Jun. 15, 1992.
Brandt et al.; Blood, vol. 79, No. 3, pp. 634-641 Feb. 1, 1992.
Flake et al.; Science, vol. 233, pp. 776-778; Aug. 15, 1986.
Bernstein et al.; Blood, vol. 77, pp. 2316-2321; Jun. 1, 1991.
Papayannopoulou, et al.; Blood, vol. 78, pp. 1403-1412; Sep. 15, 1991.
Brandt John E.
Hoffman Ronald
Srour Edward
Zanjani Esmail
Elliott George C.
Indiana University Foundation
Rowland Bertram I.
Sherwood Pamela J.
LandOfFree
Human stem cell compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Human stem cell compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human stem cell compositions and methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2255025