Human Smad3 and Smad4 are sequence-specific transcription activa

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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4353201, 536 241, C12Q 168

Patent

active

061000329

ABSTRACT:
Two human Smad proteins (Smad3 and Smad4) specifically recognize an identical 8 bp palindromic sequence (GTCTAGAC) (SEQ ID NO: 1). Tandem repeats of this palindrome confer striking TGF-.beta. responsiveness to a minimal promoter. This responsiveness is abrogated by targeted deletion of the cellular Smad4 gene. DNA molecules comprising the 8 bp palindromic sequence can be used in assays for drug screening and diagnostically.

REFERENCES:
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patent: 5712097 (1998-01-01), Kern et al.
Kinzler et al. "Whole genome PCR: application to the identiification of sequences bound by gene regulatory proteins" Nucleic Acids Research vol. 17, No. 10, 1989, pp. 3645-3853.
Arnold R. Oliphant et al. "Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein" Molecular and Cellular Biology, Jul. 1989, pp. 2944-2949.

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