Human serum albumin crystals and method of preparation

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530362, 530364, 530387, 530422, 427 2, 428408, 428420, 4284782, C07K 324, C07K 328, C07K 1500

Patent

active

048332330

ABSTRACT:
HSA crystals are provided in the form of tetragonal plates having the space groups P42.sub.1 2, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by a hanging drop method wherein a precipitant solution containing PEG and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concentration of reagents, pH and other parameters are controlled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x-ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies.

REFERENCES:
patent: 3415804 (1968-12-01), Polson
patent: 3790552 (1974-02-01), Johnson et al.
patent: 3869436 (1975-03-01), Falksveden
patent: 4164496 (1979-08-01), Hao
patent: 4177188 (1979-12-01), Hansen
patent: 4197238 (1980-04-01), Murata et al.
patent: 4489133 (1984-12-01), Kornberg
patent: 4668584 (1987-05-01), Uzgiris et al.
Vol. A of International Tables for Crystallography, Hahn, 1983, 422, No. 90.
J. Mol. Biol. (1974), 83, 551-555, McClure et al.
J. Biol. Chemistry, 251, No. 10, (1976), Rao et al.
Advances in Protein Chemistry, 37 (1985), pp. 180-181, Peters (Anfinsen et al. editors).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Human serum albumin crystals and method of preparation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Human serum albumin crystals and method of preparation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human serum albumin crystals and method of preparation will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1731498

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.