Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin
Patent
1987-08-20
1989-05-23
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Albumin
530362, 530364, 530387, 530422, 427 2, 428408, 428420, 4284782, C07K 324, C07K 328, C07K 1500
Patent
active
048332330
ABSTRACT:
HSA crystals are provided in the form of tetragonal plates having the space groups P42.sub.1 2, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by a hanging drop method wherein a precipitant solution containing PEG and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concentration of reagents, pH and other parameters are controlled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x-ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies.
REFERENCES:
patent: 3415804 (1968-12-01), Polson
patent: 3790552 (1974-02-01), Johnson et al.
patent: 3869436 (1975-03-01), Falksveden
patent: 4164496 (1979-08-01), Hao
patent: 4177188 (1979-12-01), Hansen
patent: 4197238 (1980-04-01), Murata et al.
patent: 4489133 (1984-12-01), Kornberg
patent: 4668584 (1987-05-01), Uzgiris et al.
Vol. A of International Tables for Crystallography, Hahn, 1983, 422, No. 90.
J. Mol. Biol. (1974), 83, 551-555, McClure et al.
J. Biol. Chemistry, 251, No. 10, (1976), Rao et al.
Advances in Protein Chemistry, 37 (1985), pp. 180-181, Peters (Anfinsen et al. editors).
Glenn Charles E. B.
Manning John R.
Schain Howard E.
Sheehan William J.
The United States of America as represented by the Administrator
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