Human requiem

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 6, 435 29, 4353201, 536 231, C12P 2102, C07H 2104, C12Q 168, C12N 1563

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059196602

ABSTRACT:
Human REQUIEM polypeptides and DNA (RNA) encoding such REQUIEM and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such REQUIEM for the treatment of a susceptibility to viral infection, tumorogenesis and to diseases and defects in the control embryogenesis and tissue homeostasis, and the nucleic acid sequences described above may be employed in an assay for ascertaining such susceptibility. Antagonists against such REQUIEM and their use as a therapeutic to treat Alzheimer's disease, Parkinson's disease, rheumatoid arthritis, septic shock, sepsis, stroke, CNS inflammation, osteoporosis, ischemia reperfusion injury, cell death associated with cardiovascular disease, polycystic kidney disease, apoptosis of endothelial cells in cardiovascular disease, degenerative liver disease, MS, ALS, cererbellar degeneration, ischemic injury, myocardial infarction, AIDS, myelodysplastic syndromes, aplastic anemia, male pattern baldness, and head injury damage are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to mutations in the nucleic acid sequences and altered concentrations of the polypeptides. Also disclosed are diagnostic assays for detecting mutations in the polynucleotides encoding REQUIEM polypeptide and for detecting altered levels of the polypeptide in a host.

REFERENCES:
Gabig, T.G., et al., "Requiem: A Novel Zinc Finger Gene Essential for Apoptosis in Myeloid Cells", J. Biol. Chem., 269(47):29515-29519, (1994).
GenBank Accession No. U10435.
GenBank Accession No. T13371 polynucleotide.
GenBank Accession No. T13371 polypeptide.
GenBank Accession No. D78482.
Gabig et al. Blood 84(10 Suppl. 1) 366A Abst. #14 48, 1994.
Gabig et al. J Biol Chem 269(47) 29515-29519, 1994.
Buchman et al Nucleic Acids Res. 20(21) 5579-5585, 1992.

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