Human phospholipase activating protein and methods for diagnosis

Details Human phospholipase activating protein and methods for diagnosis Human phospholipase activating protein and methods for diagnosis

1993-11-04

1994-11-22

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435198, 435 692, C12N 1500, C12N 920, C12P 2106

Patent

active

053670639

ABSTRACT:
The invention provides methods for detecting elevated levels of phospholipase A.sub.2 activating protein in persons suspected of having rheumatiod arthritis to thereby indicate the presence of rheumatoid arthritis in the person comprising the steps of providing a sample of body fluid or tissue from said person; contacting the sample with an antibody specific for phospholipase A.sub.2 activating protein such that the antibody binds with phospholipase A.sub.2 activating protein in the sample; detecting the antibody thereby indicating the presence of phospholipase A.sub.2 activating protein, whereby elevated levels of phospholipase A.sub.2 activating protein in the sample as compared with levels found in persons not having rheumatoid arthritis indicates the presence of rheumatoid arthritis in the person. Kits and reagents for detecting rheumatoid arthritis are also provided.

REFERENCES:
Bomalaski et al., "Rheumatoid Arthritis Synovial Fluid Phospholipase A.sub.2 Activating Protein (PLAP) Stimulates Human Neutrophil Degranulation and Superoxide Ion Production", Agents & Actions vol. 27, Nos. 3/4: 425-427 (1989).
Clark et al., "Tumour Necrosis Factor (Cachectin) Induces Phospholipase A.sub.2 Activating and Synthesis of a Phospholipase A.sub.2 -Activating Protein in Endothelial Cells", Adv. Exp. Med. Biol. vol. 275: 125-144 (1990).
Clark et al., "Indentification and Isolation of a Mammalian Protein Which Is Antigenically and Functionally Related to the Phospholipse A.sub.2 Stimulatory Peptide Melittin", J. Biol. Chem. vol. 262 No. 9: 4402-4406 (1987).
Bomalaski et al., "A Phospholipase A.sub.2 -Activating Protein (PLAP) Stimulates Human Neutrophil Aggregation & Release of Lysosomal Enzymes, Superoxide, and Eicosanoids", J. of Immunol. vol. 142 No. 11: 3957-3962 (1989).
Heikkila et al., Nature vol. 328: 445-449 (1987).
Green et al., "Circulating Phospholipase A.sub.2 Activity Associated with Sepsis and Septic Shock is Indistinguishable from that Associated with Rheumatoid Arthritis", Inflammation vol. 15, No. 5: 355-367 (1991).
Bomalaski et al., J. Immunol. vol. 145 No. 10: 3391-3397 (1990).

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