Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-05
1998-07-28
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 435 792, 435 794, 436506, 436509, 436518, G01N 33543, G01N 33564, G01N 33573
Patent
active
057861544
ABSTRACT:
The invention provides methods for detecting elevated levels of phospholipase A.sub.2 activating protein in persons suspected of having rheumatoid arthritis to thereby indicate the presence of rheumatoid arthritis in the person comprising the steps of providing a sample of body fluid or tissue from said person; contacting the sample with an antibody specific for phospholipase A.sub.2 activating protein such that the antibody binds with phospholipase A.sub.2 activating protein in the sample; detecting the antibody thereby indicating the presence of phospholipase A.sub.2 activating protein, whereby elevated levels of phospholipase A.sub.2 activating protein in the sample as compared with levels found in persons not having rheumatoid arthritis indicates the presence of rheumatoid arthritis in the person. Kits and reagents for detecting rheumatoid arthritis are also provided.
REFERENCES:
patent: 4916056 (1990-04-01), Brown, III et al.
patent: 5153166 (1992-10-01), Jain et al.
Pietsch, Borner and Tschopp, TIBS, 1993, 18, 292-293.
Clarke, et al., "Inhibition of phospholipase A.sub.2 activation and arachidonic acid release by phospholipase A.sub.2 activating protein (PLAP) antisense oligodeoxynucleotide," The Faseb Journal, Mar. 15, 1988, 2:4, Abstract No. 1436, A543.
Bomalaski, et al., "Inhibition of phospholipase A.sub.2 activation and prostaglandin release by phospholipase A.sub.2 activating protein (PLAP) antisense oligodeoxynucleotides," Arthritis & Rheumatism, Sep. 1990, 33:9 (supp) Abstract No. 151, S35.
Bomalaski, J.S. et al., "Monosodium Urate Crystals Stimulate Phospholipase A.sub.2 Enzyme Activities and the Synthesis of a Phospholipase A.sub.2 -Activating Protein", The J. of Immunol. 1990, 145, 3391-3397.
Bomalaski et al., "Rheumatoid Arthritis Synovial Fluid Phospholipase A.sub.2 Activating Protein (PLAP) Stimulates Human Neutrophil Degranulation and Superoxide Ion Production", Agents & Actions 1989, 27 (3/4), 425-427.
Bomalaski et al., "A Phospholipase A.sub.2 -Activating Protein (PLAP) Stimulates Human Neutrophil Aggregation & Release of Lysosomal Enzymes, Superoxide, and Eicosanoids", J. of Immunol. 1989, 142 (11), 3957-3962.
Clark et al., "Tumour Necrosis Factor (Cachectin) Induces Phospholipase A.sub.2 Activating and Synthesis of a Phospholipase A.sub.2 -Activating Protein in Endothelial Cells", Adv. Exp. Med. Biol. 1990, 275, 125-144.
Clark et al., "Identification and Isolation of a Mammalian Protein Which is Antigenically and Functionally Related to the Phospholipase A.sub.2 Stimulatory Peptide Melittin", J. Biol. Chem. 1987, 262(9), 4402-4406.
Green et al., "circulating Phospholipase A.sub.2 Activity Associated with Sepsis and Septic Shock is Indistinguishable from that Associated with Rheumatoid Arthritis", Inflammaizon 1991, 15 (5), 355-367.
Heikkila, R. et al., "A c-myc Antisense Oligodeoxynucleotide Inhibits Entry into S Phase but not Progress from G.sub.0 to G.sub.1 ", Nature 1987, 328, 445-449.
Young et al., "Efficient Isolation of Genes by Using Antibody Probes", PNAS USA 1983, 80, 1194-1198.
Bomalaski et al, J. Lab. Clin. Med. 116 (6), 814-825, 1990.
Clark et al, Biochem J., 250, 125-132, 1988.
P. Tijssen, Practice and Theory of Enzyme Immunoassay, Elsevier Science Publishing Company, Inc., 1985, pp. 329-341.
Bomalaski John S.
Clark Mike A.
Shorr Robert
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