Human phospholipase A.sub.2 and related nucleic acid compounds

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 691, 435196, 530350, C12N 916

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active

061035106

ABSTRACT:
The invention provides a novel phospholipase A.sub.2 enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade.

REFERENCES:
patent: 5045462 (1991-09-01), Menez et al.
patent: 5328842 (1994-07-01), Chiou et al.
Nalefski et al. (1994) "Delineation of Two Functionally Distinct Domains of Cytosolic Phospholipase A2, a Regulatory Ca2+-dependent Lipid-binding Domain and a Ca2+-independent Catalytic Domain" J. Biol. Chem. 269/27:18239-49.
Nalefski et al. (1996) "Cytosolic Phospholipase A2--Chicken" GB/EMBL/DDBJ Database, Accession No. I50699.
Leslie et al. (1988) "Properties and purification of an arachidonoyl-hydrolyzing phospholipase A2 from a macrophage cell line, RAW 264.7" Biochem. Biophys. Acta 963:476-92.
Molecular weight calculation of polypeptide encoded by SEQ ID No:2 using Intelligenetics PEP-Polypeptide Analysis System Version 5.4. Jan. 13, 1999 .

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