Human parathyroid hormone muteins and production thereof

Details Human parathyroid hormone muteins and production thereof Human parathyroid hormone muteins and production thereof

1996-10-18

1999-01-05

Walsh, Stephen

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4353201, 4352523, 435325, 530324, 536 2351, 536 241, C12N 1516, C07K 14635

Patent

active

058561382

ABSTRACT:
Disclosed are (1) a human parathyroid hormone mutein which comprises at least one modification selected from the group consisting of (i) deletion of 3 to 6 amino acid residues on the N-terminal side in the amino acid sequence of human parathyroid hormones, (ii) substitution of another lipophilic amino acid residue for at least one methionine residue in the amino acid sequence, and (iii) substitution of a cysteine residue for one amino acid residue within the region of amino acid residue Nos. 34 to 47 in the amino acid sequence; (2) a recombinant DNA having a nucleotide sequence coding for the human parathyroid hormone mutein described in (1); (3) a vector containing the recombinant DNA described in (2); (4) a vector in which the recombinant DNA described in (2) is inserted into a region controlled by an E. coli T7 promoter; (5) a transformant which is transformed by the recombinant DNA described in (2); and a process for producing a human parathyroid hormone mutein which comprises cultivating the transformant described in (5) in a culture medium.

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