Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-11-03
1998-04-28
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, 435174, 536 231, 536 243, 536 2432, 536 2433, 536 266, C12Q 170, C12Q 168, C12P 1934, C07H 2104
Patent
active
057442991
ABSTRACT:
The present invention is a method for evaluating a biological sample for the presence or absence of human parainfluenza virus 1 (HPIV-1) infection and for the quantitation of HPIV-1. This method comprises the steps of isolating RNA from a biological sample, creating cDNA from the isolated RNA, exposing the cDNA to a pair of oligonucleotide primers selected from sequences from the group consisting of SEQ ID NOs:1-9 and inverse complements of SEQ ID NOs:1-9 under conditions in the which the primers will amplify a human parainfluenza-1 sequence if the human parainfluenza-1 virus is present, and determining whether the amplified sequence is present by EHA. The present invention is also a kit for the detection and quantitation of parainfluenza virus 1 and a kit for the detection of parainfluenza virus-1, 2 and 3 genomic RNA.
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Fan Jiang
Henrickson Kelly J.
Jones W. Gary
MCW Research Foundation
Rees Dianne
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