Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
1997-01-02
2001-01-30
Romeo, David S. (Department: 1647)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C530S300000, C530S350000, C530S868000
Reexamination Certificate
active
06180602
ABSTRACT:
TECHNICAL FIELD
The present invention relates to human cDNA derived from mRNA expressed in human cells, TGF-beta superfamily protein encoded thereby, the vectors containing the cDNA, and a process for utilizing such proteins or DNA for therapeutic purpose as immunosuppressive agents. The human cDNA of the present invention can be used as a probe for gene diagnosis and as gene resources for mass production of the protein encoded thereby. The protein of the present invention can be used as medicine such as a therapeutic agent for autoimmune diseases, or as an antigen for preparing the antibodies against the protein. The cDNA vector of the invention facilitates preparation of probes and expression of the protein.
BACKGROUND ART
Human cells produce many secretory proteins involved in cell growth and cell differentiation. TGF-beta superfamily proteins, which belong to the secretory proteins, have attracted a great deal of attention as a potential therapeutic agent because of their diverse biological activities [Massague, J., Annu. Rev. Cell Biol. 6: 597-641, 1988]. For example, TGF-beta 2, activin, inhibin, and bone morphogenetic proteins (BMP) are known as members of TGF-beta superfamily showing various biological activities including promotion and inhibition of cell growth, promotion and inhibition of cell differentiation, as well as promotion and inhibition of other cell functions. Thus these proteins have been investigated for using as medicine such as therapeutic agents for wound-healing, bone-related disease, anti-inflammation, and autoimmune diseases.
The carboxyl terminal of about 100 to 140 amino acid residues in TGF-beta superfamily proteins contains a characteristic amino acid sequence in which the position of seven cysteines is conserved. It is expected that there are many novel TGF-beta superfamily proteins playing an important role in a living cell.
Immunosuppressive agents such as steroid, cyclosporin, and FK506 have been used for therapy of autoimmune diseases or during an organ transplantation. However these compounds cause side effects, so that a new type of immunosuppressive agent has been desired.
DISCLOSURE OF INVENTION
It is therefore an object of the present invention to provide a human novel cDNA encoding TGF-beta superfamily protein, the protein encoded thereby, and a process for utilizing such proteins or DNA for therapeutic purpose as immunosuppressive agents.
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Kato Seishi
Kobayashi Midori
Oh Suwan
Ohmori Hitoshi
Saeki Mihoro
Foley & Lardner
Romeo David S.
Sagami Chemical Research Center
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