Human Myt1 protein and chimeric proteins derived therefrom

Details Human Myt1 protein and chimeric proteins derived therefrom Human Myt1 protein and chimeric proteins derived therefrom

1998-04-27

2000-01-25

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 15, 435194, C12Q 168, C12Q 148, C12N 912

Patent

active

06017708&

ABSTRACT:
The cDNA of the human Myt1 kinase (Myt1Hu), which catalyzes phosphorylation of Cdc2 on Thr 14 and Tyr 15, has been cloned and sequenced. The invention provides isolated, purified and structurally defined DNA encoding Myt1Hu, isolated and purified Myt1Hu protein, a method for making Myt1Hu protein by expressing the DNA encoding Myt1Hu and methods for measuring levels of Myt1Hu in RNA or of Myt1Hu protein in a cell sample. Also provided are chimeric proteins having Cdc2 kinase activity.

REFERENCES:
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Honda et al. (1993) "Dephosphorylation of human p34.sup.cdc2 kinase on both Thr-14 and Tyr-15 by human phosphatase" FEBS letters, 318(3):331-334.
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Parker, L.L. and Piwnica-Worms, H. (1992) "Inactivation of the p34.sup.cdc2 -cyclin B complex by the human WEE 1 Tyrosine kinase" Science, 257:1955-1957.
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