Human myeloperoxidase and its therapeutic application

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4353201, 536 232, C12N 908, C12N 1553, C12N 1585

Patent

active

058467998

ABSTRACT:
A method of preparing biologically active human myeloperoxidase including the steps of preparing a vector for the expression of human myeloperoxidase. The vector includes plasmid pNIV2703. The plasmid includes a HindIII-SnaBI, HindIII-EcoRV or HindIII-HpaI cassette, the cassette including a complete DNA sequence for hMPO as shown in FIG. 1. CHO cells are transformed with the vector. The cells are cultured. The biologically active human myeloperoxidase is recovered.

REFERENCES:
patent: 5049493 (1991-09-01), Khosla et al.
Morishita et al, The Journal of Biological Chemistry, vol. 262, pp. 3844-3851 (1987).
Matsuura et al, J. Gen. Virol, vol. 68, pp. 1233-1250 (1987).
Connors et al, DNA, vol. 7, pp. 651-661, (1988).
Structural and biological properties of human recombinant myeloperoxidase produced by Chinese hamster ovary cell lines, Moguilevsky et al., Eur. J. Biochem. 197:605-614.
Multiple Species of Myeloperidase Messenger RNAs Produced by Alternative Splicing and Differential Polyadenylation, Hashinaka et al., Biochemistry 1988, 27, 5906-5914.
Spectral and Enzymatic Properties of human REcombinant Myeloperoxidase: Comparison with teh Mature Enzyme, Jacquet et al., Archives of Biochmeistry and Biophysics, vol. 291, No. 1, Nov. 15, 1991, pp. 132-138.
Myeloperoxidase is synthesized as larger phosphorylated precursor, The EMBO Jornal, vol. 3, No. 11, 1984, pp. 2671-2676.
Characterization of cDNA clones for human myeloperoxidase:predicted amino acid sequence and evidence for multiple mRNA species, Johnson et al., Nucleic Acids Research, vol. 15, No. 5, 1987.
Identification and Properties of an Oxoferryl Structure in Myeloperoxidase Compound II, Oertling et al., Biochemistry 1988, vol. 27, pp. 5395-5400.
Candidicidal Activity of the Human Recombinant Myeloperoxidase System, Tournay et al., Antimicrobial Agents and Chemotherapy, Jan. 1993, vol. 37, No. 1.
Lethal oxidative damage to human immunodeficiency virus by human recombinant myeloperoxidase, Moguilevsky et al., FEBS Letters, vol. 302, No. 3, pp. 209-212.
Expression of a Synthetic Gene for Horseradish Peroxidase C in Escherichia coli and Folding and Activation of the REcombinant Enzyme with Ca.sup.2 and Heme, Smith et al., The Journal of Biochemiustry, vol. 265, No. 22, Aug. 5, 1990, pp. 13335-13343.
Induction of nuetralizing antibodies by varicella-zoster virus gpII glycoprotein expressed from recombinant vaccinia virus, Massaer et al., Journal of General Virology 1993, vol. 74, pp. 491-494.
Expression of the human apolipoprotein AI gene fused to the E. coli gene for .beta.-galactosidase, Lorenzetti et al., FEBS, vol. 194, No. 2 Jan. 1986.
Expression of Cloned Human Haptoglobin and .alpha..sub.1 -Antitrypsin Complementary DNAs in Saccharomyces cerevisiae, Van Der Straten et al., DNA, vol. 5, No. 2, 1986, pp. 129-136.
High-level Production of Fully Active Human .alpha.1-Antitrypsin in Escherichia coli, Johansen et al., Mol. Bio. Med. 1987, vol. 4, pp. 291-305.
Selection of Mutations that Increase .alpha.1-Antitrypsin Gene Expression in Excherichia coli, Sutiphong et al., Mol. Biol. Med. 1987, vol. 4, pp. 307-322.
Host System Pointers, Van Berunt, Biotechnology vol. 5, Jul. 1987, p. 664.
Synthesis and Localization of Myeloperoxidase Protein in Transfected BHK Cells, Cully et al., Experimental Cell Research 180, 1989 pp. 440-450.
Effect of nucleotide s=equences directly downstream from teh AUG on teh expression of bovine somatrotropine in E. coli, Tomich et al., Nucleic Acids REs. Apr. 25, 1989, vol. 17, No. 8, pp. 3179-3197 (Abstract Only).
Effect of von Willebrand factor coexpression on teh systhesis and secretion of factor VIII in Chinese hamster ovary cells, Kaufman et al., mol. Cell. Biol. Mar. 1989, vol. 9, No. 3, pp. 1233-1242 (Abstract Only).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Human myeloperoxidase and its therapeutic application does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Human myeloperoxidase and its therapeutic application, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human myeloperoxidase and its therapeutic application will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-176421

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.