Human-mouse hybrid cell line expressing monocyte-macrophage prop

Details Human-mouse hybrid cell line expressing monocyte-macrophage prop Human-mouse hybrid cell line expressing monocyte-macrophage prop

1985-11-13

1990-11-13

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351722, 435948, 435 7021, 935 95, 935 96, 935106, 935108, 935110, 935111, C12N 528

Patent

active

049701622

ABSTRACT:
Human leukocytes, which contain monocytes and neutrophils that exhibit chemotaxis to N-formylmethionine-leucine-phenylalanine (FMLP), were fused with the mouse macrophage RAW264-TG3 cell line which exhibits chemotaxis to endotoxin-activated mouse serum (EAMS) but not to FMLP. From such fusions twelve cell lines were isolated, all of which migrated to EAMS. Four of the cell lines also exhibited chemotaxis to FMLP, and of these cell lines only one, WBC264-9, retained the capacity to migrate to FMLP after culture for 20 or more passages. WBC264-9 exhibits chemotaxis to FMLP and provides a novel system to investigate attractant-specific biochemical reactions necessary for chemotaxis.

REFERENCES:
Aksamit, R. R. et al., "Chemotaxis by Mouse Macrophage Cell Lines", J. Immunol., 126(6): 2194-2199, Jun., 1981.
Yamamoto, S. et al., "Macrophage-Like Chemotactic Hybridomas Active for Various Chemotactic Factors", Biomedical Research, 5(2): 91-100, 1984.
Harvath, L. et al., "Oxidized N-Formylmethionyl-Leucyl-Phenylalanine: Effect on the Activation of Human Monocyte and Neutrophil Chemotaxis and Superoxide Production", J. Immunol., 133(3): 1471-1476, Sep., 1984.
Backlund et al., Proc. Natl. Acad. Sci. USA, 82:2637-41 (1985).
Yamamoto et al., Immunogenetics, 19:519-526 (1984).
Aksamit, FASEB Abstract, Apr. 1985.

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