Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1986-07-14
1988-02-09
Tarcza, John E.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4351722, 435 68, 435948, 530387, 436548, 935100, 935 92, 935101, 424 85, C12N 500, C12N 1500, C12P 2100, A61K 39395
Patent
active
047242116
ABSTRACT:
A method is described for separating fused cells, resulting from fusion of human cells known to produce a specific antibody or a specific lymphokine with malignant human partner cells, from the said partner cells which comprises addition of specific antiserum capable of identifying antigenic specificities unique to the clone and non-reactive with the non-fused partner cells. After reaction of the fused cell with the antiserum, the reaction product is separated within 24 hours by indirect rosetting.
One cell line, ATCC HB-8143, secreted both IgG and IgM monoclonal antibodies, both antibodies having specificity to human breast carcinoma and being highly selective therefor.
REFERENCES:
patent: 4377513 (1983-03-01), Sugimoto et al.
patent: 4434230 (1984-02-01), Ritts, Jr.
patent: 4522918 (1985-06-01), Schlom et al.
Schlom et al., "Generation of Human Monoclonal Antibodies Reactive with Human Mammary Carcinoma Cells", Proceedings of the National Academy of Sciences, 77 (11), pp. 6841-6845, (1980).
Mesa-Tejada et al., "Detection in Human Breast Carcinomas of an Antigen Immunologically Related to a Group-Specific Antigen", Proceedings of the Natonal Academy of Sciences, 75 (3), pp. 1529-1533, (1978).
Leung et al., "Frequency of Association of Mammary Tumor Glycoprotein Antigen and Other Markers with Human Breast Tumors", Cancer Research, 39, pp. 2057-2061, (1979).
Sheikh et al., "Ductular Carcinoma of the Breast: Serum Antibodies to Tumor-Associated Antigens", Cancer, 44, pp. 2083-2089, (1979).
Nowinski et al., "Human Monoclonal Antibody Against Forssman Antigen", Science, 210, pp. 537-539, (1980).
Howard et al., "A Method for Distinguishing Benign from Malignant Breast Lesions Utilizing Antibody Present in Normal Human Sera", Cancer, 43, pp. 2279-2287, (1979).
Medical University of South Carolina
Tarcza John E.
LandOfFree
Human monoclonal antibodies and lymphokines and cell lines produ does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Human monoclonal antibodies and lymphokines and cell lines produ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human monoclonal antibodies and lymphokines and cell lines produ will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1283925