Human marrow stromal cell lines which sustain hematopoiesis

Details Human marrow stromal cell lines which sustain hematopoiesis Human marrow stromal cell lines which sustain hematopoiesis

2006-10-31

2006-10-31

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

C435S325000, C435S404000, C435S070100, C435S372000, C435S405000

Reexamination Certificate

active

07129086

ABSTRACT:
Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1α, and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.

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